Lesbian submissive play is much the same as it would be when submitting to a man, but rather than servicing a Master, the sexual and submissive activities are focused on a woman known as the Mistress. Slaves may be hired by a single Mistress, or a couple may hire a slave, requiring her to service both the Master and the Mistress.

Another situation for lesbian submissive activities is when multiple slaves are visiting at the same time. Depending on the Master, slaves may be required to perform lesbian activities with each other.

Lesbian activities include such things as kissing, cuddling, finger fucking, oral sex, using toys or other lesbian sexual acts.